Serine dehydratase (SDH)

1. Serine dehydratase (SDH) catalyzes PLP-dependent dehydration of L-Ser to pyruvate (Pyr).

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2. Crystal structure of SDH complexed with PLP and O-methyl-L-serine was determined.

SDH containing PLP-O-methyl-L-serine

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3. Catalytic mechanism based on the crystal structures and site-directed mutagenesis

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Publications:

● Ogawa, H., Takusagawa, F., Wakaki, K. Kishi, H., Eskandrian, M.R., Kobayashi, M., Date, H. Huh, N.H. & Pitot, H.C. (1999). Rat Liver Serine Dehydratase. Bacterial Expression and Two Folding Domains as Revealed by Limited Proteolysis. J. Biol. Chem. 274, 12855-12860.

● Ogawa, H., Gomi, T., Takusagawa, F., Masuda, T., Goto, T., Kan, T. & Huh, N. H. (2002). Evidence for a dimeric structure of rat liver serine dehydratase” Int J Biochem Cell Biol. 34, 533-543 (2002).

● Yamada, T., Komoto, J., Takata, Y., Ogawa, H., Pitot, H.C. & Takusagawa, F. (2003). Crystal structure of serine dehydratase from rat liver. Biochemistry 42, 12854-12865.

● Kashii, T., Gomi, T., Oya, T., Ishii, Y., Oda, H., Maruyama, M., Kobayashi, M., Masuda, T., Yamazaki, M., Nagata, T., Tsukada, K., Nakajima, A., Tatsu, K., Mori, H., Takusagawa, F., Ogawa, H. & Pitot, H.C. (2005). Some biochemical and histochemical properties of human liver serine dehydratase. Int J Biochem Cell Biol. 37, 574-589.