S-Adenosylmethionine
synthetase (MAT)
1. S-Adenosylmethionine
synthetase (MAT) catalyzes the S-adenosylmethionine (SAM) synthesis
reaction from ATP and L-Met.
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2. Crystal structure S-adenosylmethionine Synthetase complexes with various molecules and ions were determined.
S-Adenosylmethionine Synthetase with AMPPNP and
L-Met.
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3. This
enzyme has a knot in the polypeptide.
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4. Catalytic
Mechanism of MAT based on the crystal structures and site-directed mutagenesis.
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Publications:
● Takusagawa, F., Kamitori, S., Misaki, S. & Markham, G. D. (1996).
Crystal Structure of S-Adenosylmethionine Synthetase. J. Biol. Chem. 271, 136-147.
● Takusagawa,
F., Kamitori, S. & Markham, G.D. (1996). Structure and Function of
S-Adenosylmethionine Synthetase: Crystal Structure of S-Adenosylmethionine
Synthetase with ADP, Br-ADP, PPi at 2.8Å Resolution. Biochemistry 35, 2586-2596.
● Fu, Z., Markham, G. D. & Takusagawa,
F. (1996). Flexible Loop in the Structure of S-Adenosylmethionine
Synthetase Crystallized in the Tetragonal Crystal System. J. Biomol.
Struct. Dyn. 13, 727-739.
● Takusagawa, F. & Kamitori, S.
(1996). A Real Knot in Protein. J.
Am. Chem. Soc. 118, 8945-8946.
● Takusagawa, F., Fujioka, M. Spies, A. &
Schowen, R.L. (1998). S-Adenosylmethionine (AdoMet)-dependent
Methyltransferases in Comprehensive
Biological Catalysis: A Mechanistic Reference, Vol I (M. Sinnott, ed.),
Academic Press, London, pp. 1-30.
● Taylor, J.
C., Takusagawa, F. & Markham, G. D. (2002). The active site loop of
S-adenosylmethionine synthetase modulates catalytic efficiency. Biochemistry
41, 9358-9369.
● Komoto, J., Yamada, T., Takata, Y.,
Markham, G.D.& Takusagawa, F. (2004). Crystal structure of the S-adenosylmethionine synthetase ternary
complex: A novel catalytic mechanism of S-adenosylmethionine synthesis
from ATP and Met. Biochemistry 43, 1821-1831.